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Lysozyme Nanoparticle-Encapsulated Gold Nanoclusters for label-free Ratiometric Fluorescent pH Sensing: Applications to Enzyme-Substrate System and Cellular Imaging

Abstract

This study developed a simple strategy to prepare lysozyme nanoparticle-encapsulated gold nanoclusters (LysNP-AuNCs) as a dual emission probe for ratiometric sensing of pH variation in the enzyme-substrate system and live cell. The reduction of gold ion precursor with lysozyme generated lysozyme-stabilized AuNCs (Lys-AuNCs); they were demonstrated to be self-assembled into nano-aggregates during the formation of AuNCs. The aggregated Lys-AuNCs were treated with glutaraldehyde, triggering the conversion of aggregated lysozymes into green-emitting lysozyme nanoparticles (LysNPs) via the formation of C=N bonds. As a result, the AuNCs were well-distributed inside the LysNPs, as demonstrated by transmission electron microscopy and size-exclusion chromatography. The as-prepared LysNP-AuNCs exhibited two separate emission bands, large Stokes shift, excellent photostability, and salt stability. The pH-induced change in the fluorescence of LysNPs enabled LysNP-AuNCs to ratiometrically detect slight change (0.2 pH unit) in the pH range from 7.5 to 9.5. Additionally, LysNP-AuNCs were well-suited to probe enzymatic reaction-induced pH change, which was exemplified by urease-mediated hydrolysis of urea. Under a single excitation, LysNP-AuNCs were used to monitor the dual fluorescent images of HeLa cells under different pH conditions.

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Supplementary files

Publication details

The article was received on 03 Apr 2019, accepted on 16 May 2019 and first published on 17 May 2019


Article type: Paper
DOI: 10.1039/C9TB00640K
J. Mater. Chem. B, 2019, Accepted Manuscript

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    Lysozyme Nanoparticle-Encapsulated Gold Nanoclusters for label-free Ratiometric Fluorescent pH Sensing: Applications to Enzyme-Substrate System and Cellular Imaging

    C. Shanmugam, J. You, J. Xue and W. Tseng, J. Mater. Chem. B, 2019, Accepted Manuscript , DOI: 10.1039/C9TB00640K

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