Dual-channel fluorescent probe bearing two-photon activity for cell viability monitoring
The search for highly sensitive and efficient method to monitor cell viability is a high-priority target yet challenge. In this work, two chromophores, 1OC and 2OC, have been rational designed and fabricated to achieve excellent biocompatibility and two-photon activity in near-infrared region. Fortunately, thanks to the introduction of water-soluble moiety and biocompatible triazole groups linking with ether oxygen chain via “Click” reaction, the obtained 2OC displayed considerable biocompatibility and two-photon activity. Additionally, it revealed dual-channel fluorescence microscopy imaging for nucleolar and mitochondrial targeting, which can be employed for cell viability monitoring.