Identify fatty liver diverse stage by a two-photon absorption triphenylamine-based BODIPY analogues
Fatty liver disease (FLD) is one of the major health hazards against people's health, while its effective and accurate detection during different phase is still problematic. Herein, four BODIPY analogues (01B-04B) with different donor/acceptor group were rational designed for such purpose. Within them, the photophysical and theoretical study demonstrated that 03B has an obvious intramolecular charge transfer (ICT) with the two-photon action cross-sections up to 198 GM in the near-infrared region. The initial in vitro assessment indicated that 03B could interact with the lipid substances with significant fluorescence enhancement. Due to its high biocompatibility, lipid-affinity and sensitivity, 03B can be targeted 3T3-L1 model cells at different FLD induced stages. Further in vivo study was demonstrated that 03B could clearly distinguish FLD tissue and display the lesion in situ. These results offered a promising optical tool for FLD evaluation among different phases and monitoring dynamics at sub-cellular level.