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Constructing peptide-based artificial hydrolase with customized selectivity


Peptide-based materials are promising building blocks for the fabrication of artificial enzymes due to their proteic nature and tailorable structure. However, it is still a big challenge to create artificial enzymes with designable selectivity. In this study, molecular imprinting strategy was combined with the self-assembling of Fmoc-FFH to create an artificial hydrolase with specific selectivity. By using p-NPA as the imprinting template, we found that the imprinted polymer that coated on the surface of the catalytic peptide nanofibers provided an specific binding site to p-NPA, and hence enhancing the catalytic efficiency of the artificial hydrolase. Furthermore, we confirmed that the substrate selectivity of this artificial enzyme can be customized through changing imprinting template. The obtained catalyst is recyclable and exhibits higher catalytic activity in a wider reaction temperature and pH due to the introduction of polymer. This study provides a new approach to construct enzyme mimics with customized substrate selectivity based on the peptide-based material platform.

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Publication details

The article was received on 28 Feb 2019, accepted on 30 Apr 2019 and first published on 08 May 2019

Article type: Paper
DOI: 10.1039/C9TB00408D
J. Mater. Chem. B, 2019, Accepted Manuscript

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    Constructing peptide-based artificial hydrolase with customized selectivity

    M. Zhu, M. Wang, W. Qi, R. Su and Z. He, J. Mater. Chem. B, 2019, Accepted Manuscript , DOI: 10.1039/C9TB00408D

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