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An immunochromatographic strip sensor for sildenafil and its analogues

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Abstract

In this study, a new hapten of sildenafil (Sild) was successfully synthesized and a sensitive monoclonal antibody (mAb) against Sild was produced based on this new hapten. The subclass of the mAb was IgG2a, and the half-maximal inhibitory concentration (IC50) of the mAb was 0.53 ng mL−1. Next, an immunochromatographic assay (ICA) was established for detecting Sild and its analogues in functional foods, where the visual detection limit (vLOD) and cut-off values were 0.5 and 20 μg kg−1, respectively. With the aid of a strip scan reader, the ICA can measure Sild with an LOD of 0.7 μg kg−1 and a line range of detection between 1.40 and 13.11 μg kg−1. The whole test process takes only 15 min. Therefore, the ICA provides a useful tool for the on-site detection and rapid initial screening of Sild in functional food samples.

Graphical abstract: An immunochromatographic strip sensor for sildenafil and its analogues

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Publication details

The article was received on 09 Feb 2019, accepted on 08 Mar 2019 and first published on 09 Mar 2019


Article type: Paper
DOI: 10.1039/C9TB00280D
J. Mater. Chem. B, 2019, Advance Article

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    An immunochromatographic strip sensor for sildenafil and its analogues

    Z. Wang, X. Wu, L. Liu, L. Xu, H. Kuang and C. Xu, J. Mater. Chem. B, 2019, Advance Article , DOI: 10.1039/C9TB00280D

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