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A fluorogenic probe based on chelation–hydrolysis-enhancement mechanism for visualizing Zn2+ in Parkinson's disease models

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Abstract

Developing efficient methods for the real-time detection of Zn2+ levels in biological systems is highly relevant to improving our understanding of the role of Zn2+ in the progression of Parkinson's disease (PD). In this work, a novel Schiff base based Zn2+ fluorescent probe (ZP) was designed, synthesized and systematically investigated. A significant turn-on effect on ZP upon the addition of Zn2+ was observed, accompanied by a blue-shift of the fluorescence spectra. ZP is sensitive to Zn2+ and has excellent selectivity against various biologically relevant cations, anions and amino acids. The sensing mechanism of ZP was studied by 1H NMR, MS, single crystal X-ray diffraction and theoretical calculations. The results showed that the response of ZP to Zn2+ was based on the chelation–hydrolysis-enhancement process. Upon bonding, Zn2+ hydrolyzes the Schiff base to an aldehyde precursor, the resulting aldehyde further coordinates to Zn2+ to form a more stable heterobimetallic complex leading to the emission enhancement and blue-shift. ZP was applied to imaging exogenous/endogenous Zn2+ in live HeLa cells. Furthermore, we successfully measured the Zn2+ levels using in vitro PD models, which provided a visualization method to better understand the relationship between Zn2+ levels and PD development.

Graphical abstract: A fluorogenic probe based on chelation–hydrolysis-enhancement mechanism for visualizing Zn2+ in Parkinson's disease models

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Publication details

The article was received on 24 Dec 2018, accepted on 23 Jan 2019 and first published on 24 Jan 2019


Article type: Paper
DOI: 10.1039/C8TB03343A
Citation: J. Mater. Chem. B, 2019, Advance Article

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    A fluorogenic probe based on chelation–hydrolysis-enhancement mechanism for visualizing Zn2+ in Parkinson's disease models

    G. Zhang, Y. Zhao, B. Peng, Z. Li, C. Xu, Y. Liu, C. Zhang, N. H. Voelcker, L. Li and W. Huang, J. Mater. Chem. B, 2019, Advance Article , DOI: 10.1039/C8TB03343A

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