Jump to main content
Jump to site search

Issue 8, 2019
Previous Article Next Article

A novel near-infrared fluorescent probe for detecting intracellular alkaline phosphatase and imaging of living cells

Author affiliations

Abstract

Development of novel near-infrared (NIR) fluorescent probes for the monitoring of active substances in living organism is desirable in biological studies. Herein, we designed a novel NIR MTR fluorophore which has a longer emission wavelength, greater Stokes shift and higher quantum yield than the classic hemicyanine NIR fluorophore. The synthesized MTR-derived NIR probe (MTR-P) is highly selective and sensitive to alkaline phosphatase (ALP) activity. In the presence of ALP, MTR-P exhibited increased fluorescence signal by up to 56 fold at 723 nm, and it was determined to be 0.042 U Lāˆ’1. In addition, the mechanism of the MTR-P probe was further examined by HPLC, mass spectrometry and DFT/TDDFT calculations. The NIR probe MTR-P was successfully applied to measure the levels of alkaline phosphatase in different types of cells by fluorescence imaging.

Graphical abstract: A novel near-infrared fluorescent probe for detecting intracellular alkaline phosphatase and imaging of living cells

Back to tab navigation

Supplementary files

Publication details

The article was received on 09 Dec 2018, accepted on 24 Jan 2019 and first published on 24 Jan 2019


Article type: Paper
DOI: 10.1039/C8TB03230K
Citation: J. Mater. Chem. B, 2019,7, 1284-1291

  •   Request permissions

    A novel near-infrared fluorescent probe for detecting intracellular alkaline phosphatase and imaging of living cells

    L. Xu, X. He, Y. Huang, P. Ma, Y. Jiang, X. Liu, S. Tao, Y. Sun, D. Song and X. Wang, J. Mater. Chem. B, 2019, 7, 1284
    DOI: 10.1039/C8TB03230K

Search articles by author

Spotlight

Advertisements