Jump to main content
Jump to site search


Nanoparticle-facilitated autophagy inhibition of cancer stem cells for improved chemotherapeutic effects on glioblastoma

Abstract

Due to the rapid growth of tumor, the metabolism of tumor cells become more active, resulted in the albumin-binding proteins overexpressed for transporting of albumin as an energy and amino source. In that case, making use of nutrient transporters for drug delivery targeting to brain becomes attractive. Herein, we synthesized albumin nanoparticles by a desolvation process, modified with folic acid to enhance blood-brain-barrier (BBB) penetration and cellular uptake, and then loaded with antitumor drug paclitaxel (PTX) and autophagy inhibitor chloroquine (CQ) for combination therapy. The albumin nanoparticles could cross the BBB and target glioma cells effectively, and the combination therapy of PTX and CQ induced more cell apoptosis than PTX treatment alone in vitro. The results of autophagy role in the sensitivity of chemotherapeutic PTX to glioma cells displayed that the stemness associating genes (SOX2, POU5F1 and NANOG) of alive glioma cells increased in the presence of PTX while dropped sharply with the combination of CQ. More importantly, it was found that the combined delivery systems FA-BSA-NPPTX/CQ exhibited the most effective cell apoptosis. Our findings demonstrated that drug-loaded albumin nanoparticles could facilitate combination of chemotherapy and autophagy inhibition for effective glioma therapy.

Back to tab navigation

Supplementary files

Publication details

The article was received on 01 Dec 2018, accepted on 11 Feb 2019 and first published on 13 Feb 2019


Article type: Paper
DOI: 10.1039/C8TB03165G
Citation: J. Mater. Chem. B, 2019, Accepted Manuscript

  •   Request permissions

    Nanoparticle-facilitated autophagy inhibition of cancer stem cells for improved chemotherapeutic effects on glioblastoma

    L. Lu, X. Shen, B. Tao, C. Lin, K. Li, Z. Luo and K. Cai, J. Mater. Chem. B, 2019, Accepted Manuscript , DOI: 10.1039/C8TB03165G

Search articles by author

Spotlight

Advertisements