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Creating gradients of amyloid fibrils from the liquid–liquid interface

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Abstract

We report a method to deposit amyloid fibrils on a substrate creating gradients in orientation and coverage on demand. For this purpose, we adapt a colloidal self-assembly method at liquid–liquid interfaces to deposit amyloid fibrils on a substrate from the water–hexane interface, while simultaneously compressing it. The amyloid fibril layers orient perpendicularly to the compression, ranging from isotropic to nematic distributions. We furthermore observe reproducible transitions from a monolayer to a bilayer and from a bilayer to multilayers with increasing surface pressures. The creation of each new layer is accompanied by a systematic drop in the structural order of the system, which is however regained upon further compression. This method shows great potential for overcoming the thin-film engineering challenges associated with the manipulation of sticky amyloid fibrils, and allows their ex situ visualisation under compression at the fluid–fluid interface, a situation relevant to understand the propagation of amyloid-related diseases, their functional role in biological systems, and their potential for technological applications.

Graphical abstract: Creating gradients of amyloid fibrils from the liquid–liquid interface

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Publication details

The article was received on 10 Sep 2019, accepted on 29 Sep 2019 and first published on 02 Oct 2019


Article type: Communication
DOI: 10.1039/C9SM01826C
Soft Matter, 2019, Advance Article
  • Open access: Creative Commons BY license
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    Creating gradients of amyloid fibrils from the liquid–liquid interface

    K. B. Smith, M. Á. Fernandez-Rodriguez, L. Isa and R. Mezzenga, Soft Matter, 2019, Advance Article , DOI: 10.1039/C9SM01826C

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