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A rational approach to form disulfide linked mucin hydrogels

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Abstract

We report the design of a mucin hydrogel created using a thiol-based cross-linking strategy. By using a cross-linking reagent capable of forming hydrogen bonds and disulfide linkages within the gel network, we were able to produce mucin-based hydrogels with viscoelastic properties similar to natural mucus as measured by bulk rheology. We confirmed disulfide cross-links strongly contribute to gel formation in our system using chemical treatments to block and reduce cysteines where we found mucin hydrogel network formation was inhibited and disrupted, respectively. Particle tracking microrheology was used to investigate the kinetics and evolution of microstructure and viscoelasticity within the hydrogel as it formed. We found that the rate of gel formation could be tuned by varying the mucin to crosslinker ratio, producing network pore sizes in the range measured previously in human mucus. The results of this work provide a new, simple method for creating mucin hydrogels with physiologically relevant properties using readily available reagents.

Graphical abstract: A rational approach to form disulfide linked mucin hydrogels

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Publication details

The article was received on 23 Aug 2019, accepted on 16 Oct 2019 and first published on 17 Oct 2019


Article type: Paper
DOI: 10.1039/C9SM01715A
Soft Matter, 2019, Advance Article

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    A rational approach to form disulfide linked mucin hydrogels

    K. Joyner, D. Song, R. F. Hawkins, R. D. Silcott and G. A. Duncan, Soft Matter, 2019, Advance Article , DOI: 10.1039/C9SM01715A

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