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Issue 45, 2019
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Nuclear size changes caused by local motion of cell boundaries unfold the nuclear lamina and dilate chromatin and intranuclear bodies

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Abstract

The mechanisms by which mammalian nuclear shape and size are established in cells, and become abnormal in disease states are not understood. Here, we tracked motile cells that underwent systematic changes in cell morphology as they moved from 1-D to 2-D micro-patterned adhesive domains. Motion of the cell boundaries during cell motility caused a dynamic and systematic change in nuclear volume. Short time scales (∼1 h) distinguished the dilation of the nucleus from the familiar increase that occurs during the cell cycle. Nuclear volume was systematically different between cells cultured in 3-D, 2-D and 1-D environments. Dilation of the nuclear volume was accompanied by dilation of chromatin, a decrease in the number of folds in the nuclear lamina, and an increase in nucleolar volume. Treatment of 2-D cells with non-muscle myosin-II inhibitors decreased cell volume, and proportionately caused a decrease in nuclear volume. These data suggest that nuclear size changes during cell migration may potentially impact gene expression through the modulation of intranuclear structure.

Graphical abstract: Nuclear size changes caused by local motion of cell boundaries unfold the nuclear lamina and dilate chromatin and intranuclear bodies

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Publication details

The article was received on 16 Aug 2019, accepted on 23 Oct 2019 and first published on 25 Oct 2019


Article type: Paper
DOI: 10.1039/C9SM01666J
Soft Matter, 2019,15, 9310-9317

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    Nuclear size changes caused by local motion of cell boundaries unfold the nuclear lamina and dilate chromatin and intranuclear bodies

    A. Katiyar, V. J. Tocco, Y. Li, V. Aggarwal, A. C. Tamashunas, R. B. Dickinson and T. P. Lele, Soft Matter, 2019, 15, 9310
    DOI: 10.1039/C9SM01666J

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