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The first study on the impact of osmolytes in whole cells of high temperature-adapted microorganisms

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Abstract

The hyperthermophilic piezophile, Thermococcus barophilus displays a strong stress response characterized by the accumulation of the organic osmolyte, mannosylglycerate during growth under sub-optimal pressure conditions (0.1 MPa). Taking advantage of this known effect, the impact of osmolytes in piezophiles in an otherwise identical cellular context was investigated, by comparing T. barophilus cells grown under low or optimal pressures (40 MPa). Using neutron scattering techniques, we studied the molecular dynamics of live cells of T. barophilus at different pressures and temperatures. We show that in the presence of osmolytes, cells present a higher diffusion coefficient of hydration water and an increase of bulk water motions at a high temperature. In the absence of osmolytes, the T. barophilus cellular dynamics is more responsive to high temperature and high hydrostatic pressure. These results therefore give clear evidence for a protecting effect of osmolytes on proteins.

Graphical abstract: The first study on the impact of osmolytes in whole cells of high temperature-adapted microorganisms

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Publication details

The article was received on 16 Jun 2019, accepted on 20 Sep 2019 and first published on 27 Sep 2019


Article type: Paper
DOI: 10.1039/C9SM01196J
Soft Matter, 2019, Advance Article

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    The first study on the impact of osmolytes in whole cells of high temperature-adapted microorganisms

    M. Salvador-Castell, M. Golub, N. Martinez, J. Ollivier, J. Peters and P. Oger, Soft Matter, 2019, Advance Article , DOI: 10.1039/C9SM01196J

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