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Issue 21, 2019
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Acoustic trap-and-release for rapid assessment of cell motility

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Abstract

Functional cilia and flagella are crucial to the propulsion of physiological fluids, motile cells, and microorganisms. Motility assessment of individual cells allows discrimination of normal from dysfunctional behavior, but cell-scale analysis of individual trajectories to represent a population is laborious and impractical for clinical, industrial, and even research applications. We introduce an assay that quantifies swimming capability as a function of the variation in polar moment of inertia of cells released from an acoustic trap. Acoustic confinement eliminates the need to trace discrete trajectories and enables automated analysis of hundreds of cells in minutes. The approach closely approximates the average speed estimated from the mean squared displacement of individual cells for wild-type Chlamydomonas reinhardtii and two mutants (ida3 and oda5) that display aberrant swimming behaviors. Large-population acoustic trap-and-release rapidly differentiates these cell types based on intrinsic motility, which provides a highly sensitive and efficient alternative to conventional particle tracing.

Graphical abstract: Acoustic trap-and-release for rapid assessment of cell motility

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Publication details

The article was received on 25 Jan 2019, accepted on 01 Apr 2019 and first published on 03 Apr 2019


Article type: Paper
DOI: 10.1039/C9SM00184K
Soft Matter, 2019,15, 4266-4275

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    Acoustic trap-and-release for rapid assessment of cell motility

    M. Kim, E. Huff, M. Bottier, S. K. Dutcher, P. V. Bayly and J. M. Meacham, Soft Matter, 2019, 15, 4266
    DOI: 10.1039/C9SM00184K

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