Jump to main content
Jump to site search


Layer-by-layer assembly for immobilizing enzymes in enzymatic biofuel cells

Author affiliations

Abstract

Various methods of immobilizing enzymes on electrodes have been developed for the research of enzymatic biofuel cells (EBFCs). Among these methods, the layer-by-layer (LbL) assembly is a delicate technology that can form controllable enzyme layers and load a high density of enzyme molecules by electrostatic deposition, hydrogen bonding, or covalent bonding. In this paper, recent advancements of the LbL assembly for immobilizing enzymes in EBFCs are presented in detail. First, the LbL formation mechanism of a multilayer sensitive film on electrodes is analyzed, and the potential of the multilayer structure is also discussed. Then, the applications of enzyme immobilization by the LbL assembly in combination with various materials in EBFCs are investigated. The power density of LbL assembly-based EBFCs increases by several orders of magnitude with the widespread use of nanomaterials. As a convenient and efficient operation, the LbL assembly technology is expected to become a popular method for immobilizing enzymes in EBFCs.

Graphical abstract: Layer-by-layer assembly for immobilizing enzymes in enzymatic biofuel cells

Back to tab navigation

Publication details

The article was received on 13 Aug 2019, accepted on 10 Oct 2019 and first published on 11 Oct 2019


Article type: Review Article
DOI: 10.1039/C9SE00643E
Sustainable Energy Fuels, 2020, Advance Article

  •   Request permissions

    Layer-by-layer assembly for immobilizing enzymes in enzymatic biofuel cells

    J. Zhang, X. Huang, L. Zhang, Y. Si, S. Guo, H. Su and J. Liu, Sustainable Energy Fuels, 2020, Advance Article , DOI: 10.1039/C9SE00643E

Search articles by author

Spotlight

Advertisements