Layer-by-layer assembly for immobilizing enzymes in enzymatic biofuel cells
Various methods immobilizing enzymes on electrodes have been developed for the research of enzymatic biofuel cells (EBFCs). Thereinto, layer-by-layer (LbL) assembly is a delicate technology that can form the controllable enzyme layers and load high density enzyme molecules by electrostatic deposition. In this paper, recent advancements of LbL assembly about immobilizing enzymes in EBFCs are presented in detail. Firstly, the LbL formation mechanism of multilayer sensitive film on electrodes is analyzed, and its progressive potential of multilayer structure is also discussed. Then, the applications of enzyme immobilization by LbL assembly joining with various materials in EBFCs are investigated respectively. Due to the widespread of nanomaterials, the power density of LbL assembly-based EBFC increases by several orders of magnitude. As the convenient and efficient operation, LbL assembly technology is expected to become a popular method for immobilizing enzymes in EBFCs.