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Programmable mismatch-fueled high-efficiency DNA signal converter

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Abstract

Herein, by directly introducing mismatched reactant DNA, high-reactivity and high-threshold enzyme-free target recycling amplification (EFTRA) is explored. The developed high-efficiency EFTRA (HEEFTRA) was applied as a programmable DNA signal converter, possessing higher conversion efficiency than the traditional one with perfect complement owing to the more negative reaction standard free energy (ΔG). Once traces of input target miRNA interact with the mismatched reactant DNA, amounts of ferrocene (Fc)-labeled output DNA could be converted via the EFTRA. Impressively, the Fc-labeled output DNA could be easily captured by the DNA tetrahedron nanoprobes (DTNPs) on the electrode surface to form triplex-forming oligonucleotide (TFO) at pH = 7.0 for sensitive electrochemical signal generation and the DTNPs could be regenerated at pH = 10.0, from which the conversion efficiency (N) will be accurately obtained, benefiting the selection of suitable mismatched bases to obtain high-efficiency EFTRA (HEEFTRA). As a proof of concept, the HEEFTRA as an evolved DNA signal converter is successfully applied for the ultrasensitive detection of miRNA-21, which gives impetus to the design of other signal converters with excellent efficiency for ultimate applications in sensing analysis, clinical diagnosis, and other areas.

Graphical abstract: Programmable mismatch-fueled high-efficiency DNA signal converter

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Publication details

The article was received on 09 Oct 2019, accepted on 21 Oct 2019 and first published on 07 Nov 2019


Article type: Edge Article
DOI: 10.1039/C9SC05084A
Chem. Sci., 2019, Advance Article
  • Open access: Creative Commons BY-NC license
    All publication charges for this article have been paid for by the Royal Society of Chemistry

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    Programmable mismatch-fueled high-efficiency DNA signal converter

    X. Zhang, Z. Yang, Y. Chang, D. Liu, Y. Li, Y. Chai, Y. Zhuo and R. Yuan, Chem. Sci., 2019, Advance Article , DOI: 10.1039/C9SC05084A

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