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High frequency, calibration-free molecular measurements in situ in the living body

Abstract

Abolition of the need for end-users to perform sensor calibration proved key to the widespread use of home-glucose monitors. Motivated by this observation here we have adapted electrochemical aptamer-based (E-AB) sensors, a sensing technology that is far more general than the glucose monitor, to the problem of performing calibration-free in-vivo measurements of molecules other than glucose. Specifically, we first demonstrate the ability of E-AB sensors to achieve the accurate and precise measurement of cocaine, ATP and kanamycin in vitro in undiluted whole blood, achieving clinically relevant accuracy (better than ±20%) in undiluted whole blood without the need to calibrate individual sensors. We then demonstrate similar, calibration-free accuracy (±30%) for ATP and kanamycin measurements with sensors when placed in situ in the jugular veins of live rats over multi-hour measurements runs that achieve time resolution of seconds and concentration precision of a few micromolar.

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Publication details

The article was received on 03 Sep 2019, accepted on 05 Nov 2019 and first published on 06 Nov 2019


Article type: Edge Article
DOI: 10.1039/C9SC04434E
Chem. Sci., 2019, Accepted Manuscript
  • Open access: Creative Commons BY-NC license
    All publication charges for this article have been paid for by the Royal Society of Chemistry

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    High frequency, calibration-free molecular measurements in situ in the living body

    H. Li, S. Li, J. Dai, C. li, M. Zhu, H. Li, X. Lou, F. Xia and K. W. Plaxco, Chem. Sci., 2019, Accepted Manuscript , DOI: 10.1039/C9SC04434E

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