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Issue 43, 2019
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Two-dimensional UV spectroscopy: a new insight into the structure and dynamics of biomolecules

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Abstract

Two-dimensional (2D) spectroscopy, originally developed for nuclear magnetic resonance, has been recently extended to the infrared and visible regimes. In this technique sequences of femtosecond light pulses are used to interrogate molecular systems and show, by a double Fourier transform, the correlation between excitation and detection frequencies. Extension to the ultraviolet (UV) regime is of great interest and promises to deliver rich structural and dynamical information on biomolecules such as DNA and proteins; however, it must overcome significant technical challenges. This review summarizes the current development status of 2DUV spectroscopy. After discussing the scientific case for the technique, we introduce its basic principles and review its experimental implementations, as well as the computational tools that have been developed to model the experiments. We conclude by giving a few application examples, which highlight the potential of 2DUV spectroscopy and motivate its further development.

Graphical abstract: Two-dimensional UV spectroscopy: a new insight into the structure and dynamics of biomolecules

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Article information


Submitted
03 Aug 2019
Accepted
30 Sep 2019
First published
15 Oct 2019

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2019,10, 9907-9921
Article type
Perspective

Two-dimensional UV spectroscopy: a new insight into the structure and dynamics of biomolecules

R. Borrego-Varillas, A. Nenov, L. Ganzer, A. Oriana, C. Manzoni, A. Tolomelli, I. Rivalta, S. Mukamel, M. Garavelli and G. Cerullo, Chem. Sci., 2019, 10, 9907
DOI: 10.1039/C9SC03871J

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