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Structural analysis and cellular visualization of APP RNA G-quadruplex

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Abstract

RNA G-quadruplexes (rG4s) are emerging structural motifs that are of pivotal importance in chemistry and biology; however, the current structural information of rG4s is limited, with their folding status and functions in cells remaining elusive. Here, we develop and employ a multi-disciplinary approach to characterize the structure, formation and function of an individual rG4 of interest in vitro and in cells. We apply this strategy to a biologically important rG4 in amyloid precursor protein (APP) transcript and reveal distinct structural features of APP rG4. Notably, we visualize the formation of APP rG4 in cells using an APP-specific G-quadruplex-triggered fluorogenic hybridization (GTFH) probe and report that the regulatory role of APP rG4 in translation is dependent on rG4 thermostability, providing evidence to the existence and significance of the stable rG4 structure in gene regulation.

Graphical abstract: Structural analysis and cellular visualization of APP RNA G-quadruplex

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Publication details

The article was received on 07 Jun 2019, accepted on 21 Oct 2019 and first published on 29 Oct 2019


Article type: Edge Article
DOI: 10.1039/C9SC02768H
Chem. Sci., 2019, Advance Article
  • Open access: Creative Commons BY license
    All publication charges for this article have been paid for by the Royal Society of Chemistry

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    Structural analysis and cellular visualization of APP RNA G-quadruplex

    K. Lyu, S. Chen, C. Chan, J. Tan and C. K. Kwok, Chem. Sci., 2019, Advance Article , DOI: 10.1039/C9SC02768H

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