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Issue 35, 2019
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In vivo methylation of OLA1 revealed by activity-based target profiling of NTMT1

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Abstract

N-Terminal methyltransferase 1 (NTMT1) catalyzes the N-terminal methylation of proteins with a specific N-terminal motif after methionine removal. Aberrant N-terminal methylation has been implicated in several cancers and developmental diseases. Together with motif sequence and signal peptide analyses, activity-based substrate profiling of NTMT1 utilizing (E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine (Hey-SAM) revealed 72 potential targets, which include several previously confirmed ones and many unknowns. Target validation using normal and NTMT1 knock-out (KO) HEK293FT cells generated by CRISPR-Cas9 demonstrated that Obg-like ATPase 1 (OLA1), a protein involved in many critical cellular functions, is methylated in vivo by NTMT1. Additionally, Hey-SAM synthesis achieved ≥98% yield for SAH conversion.

Graphical abstract: In vivo methylation of OLA1 revealed by activity-based target profiling of NTMT1

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Supplementary files

Article information


Submitted
24 May 2019
Accepted
08 Aug 2019
First published
09 Aug 2019

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2019,10, 8094-8099
Article type
Edge Article

In vivo methylation of OLA1 revealed by activity-based target profiling of NTMT1

K. Jia, G. Huang, W. Wu, R. Shrestha, B. Wu, Y. Xiong and P. Li, Chem. Sci., 2019, 10, 8094
DOI: 10.1039/C9SC02550B

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