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In vivo methylation of OLA1 revealed by activity-based target profiling of NTMT1

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Abstract

N-Terminal methyltransferase 1 (NTMT1) catalyzes the N-terminal methylation of proteins with a specific N-terminal motif after methionine removal. Aberrant N-terminal methylation has been implicated in several cancers and developmental diseases. Together with motif sequence and signal peptide analyses, activity-based substrate profiling of NTMT1 utilizing (E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine (Hey-SAM) revealed 72 potential targets, which include several previously confirmed ones and many unknowns. Target validation using normal and NTMT1 knock-out (KO) HEK293FT cells generated by CRISPR-Cas9 demonstrated that Obg-like ATPase 1 (OLA1), a protein involved in many critical cellular functions, is methylated in vivo by NTMT1. Additionally, Hey-SAM synthesis achieved ≥98% yield for SAH conversion.

Graphical abstract: In vivo methylation of OLA1 revealed by activity-based target profiling of NTMT1

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Publication details

The article was received on 24 May 2019, accepted on 08 Aug 2019 and first published on 09 Aug 2019


Article type: Edge Article
DOI: 10.1039/C9SC02550B
Chem. Sci., 2019, Advance Article
  • Open access: Creative Commons BY license
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    In vivo methylation of OLA1 revealed by activity-based target profiling of NTMT1

    K. Jia, G. Huang, W. Wu, R. Shrestha, B. Wu, Y. Xiong and P. Li, Chem. Sci., 2019, Advance Article , DOI: 10.1039/C9SC02550B

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