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Structure and effective charge characterization of proteins by a mobility capillary electrophoresis based method

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Abstract

Measuring the conformations and effective charges of proteins in solution is critical for investigating protein bioactivity, but their rapid analysis remains a challenging problem. Here we report a mobility capillary electrophoresis (MCE) based method for the rapid analysis of protein stereo-structures and effective charges in different solution environments. With the capability of mixture separation, MCE measures the hydrodynamic radius of a protein through Taylor dispersion analysis and its effective charge through ion mobility analysis. The experimental results acquired from MCE are then utilized to restrain molecular dynamics simulations, so that the most probable conformation of that protein can be obtained. As proof-of-concept demonstrations, the charge states and structures of five proteins were analyzed under close to native environments. The conformation transitions and charge state variations of bovine serum albumin and lysozyme under different pH conditions were also investigated. This method is promising for high-throughput protein analysis, which could potentially be coupled with mass spectrometry for investigating protein stereo-structures and functions in top-down proteomics.

Graphical abstract: Structure and effective charge characterization of proteins by a mobility capillary electrophoresis based method

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Publication details

The article was received on 25 Apr 2019, accepted on 02 Jul 2019 and first published on 03 Jul 2019


Article type: Edge Article
DOI: 10.1039/C9SC02039J
Chem. Sci., 2019, Advance Article
  • Open access: Creative Commons BY-NC license
    All publication charges for this article have been paid for by the Royal Society of Chemistry

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    Structure and effective charge characterization of proteins by a mobility capillary electrophoresis based method

    W. Zhang, H. Wu, R. Zhang, X. Fang and W. Xu, Chem. Sci., 2019, Advance Article , DOI: 10.1039/C9SC02039J

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