Issue 34, 2019

Peptide-induced super-assembly of biocatalytic metal–organic frameworks for programmed enzyme cascades

Abstract

Despite the promise of metal–organic frameworks (MOFs) as functional matrices for enzyme stabilization, the development of a stimulus-responsive approach to induce a multi-enzyme cascade reaction in MOFs remains a critical challenge. Here, a novel method using peptide-induced super-assembly of MOFs is developed for programmed enzyme cascade reactions on demand. The super-assembled MOF particles containing different enzymes show remarkable 7.3-fold and 4.4-fold catalytic activity enhancements for the two-enzyme and three-enzyme cascade reactions, respectively, as compared with the unassembled MOF nanoparticles. Further digestion of the coiled-coil forming peptides on the MOF surfaces leads to the MOF superstructure disassembly and the programmed enzyme cascade reaction being “switched-off”. Research on these stimuli-responsive materials with controllable and predictable biocatalytic functions/properties provide a concept to facilitate the fabrication of next-generation smart materials based on precision chemistry.

Graphical abstract: Peptide-induced super-assembly of biocatalytic metal–organic frameworks for programmed enzyme cascades

Supplementary files

Article information

Article type
Edge Article
Submitted
24 Apr 2019
Accepted
20 Jul 2019
First published
22 Jul 2019
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2019,10, 7852-7858

Peptide-induced super-assembly of biocatalytic metal–organic frameworks for programmed enzyme cascades

J. Liang, F. Mazur, C. Tang, X. Ning, R. Chandrawati and K. Liang, Chem. Sci., 2019, 10, 7852 DOI: 10.1039/C9SC02021G

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