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Chemical operations on a living single cell by open microfluidics for wound repair studies and organelle transport analysis

Abstract

Single cells are increasingly recognized to be capable of wound repair that is important for our mechanistic understanding of cell biology. The lack of flexible, facile, and friendly subcellular treatment methods have hindered single-cell would repair studies and organelle transport analyses. Here we report a laminar flow based approach, we call it fluid cell knife (FCK), that is capable of precisely cutting off or treating a portion of a single cell from its remain portion in its original adherent state. Local operations of portions of a living single cell in its adherent culture state were applied to various types of cells. Temporal wound repair was sucessfully observed. Moreover, we successfully stained portions of a living single cell to measure organelle transport speed (mitochondria as a model) inside a cell. This technique opens up new avenues for cell wound repair and subcellular behavior analyses.

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Publication details

The article was accepted on 27 Nov 2018 and first published on 04 Dec 2018


Article type: Edge Article
DOI: 10.1039/C8SC05104F
Citation: Chem. Sci., 2019, Accepted Manuscript
  • Open access: Creative Commons BY-NC license
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    Chemical operations on a living single cell by open microfluidics for wound repair studies and organelle transport analysis

    S. Mao, Q. Zhang, W. Liu, Q. Huang, M. Khan, W. Zhang, C. Lin, K. Uchiyama and J. Lin, Chem. Sci., 2019, Accepted Manuscript , DOI: 10.1039/C8SC05104F

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