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Issue 8, 2019
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Simultaneous dual-colour tracking lipid droplets and lysosomes dynamics using a fluorescent probe

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Abstract

After entering a cell, most small molecule fluorescent probes are dispersed in the cytoplasm before they then accumulate in a specific organelle or subcellular zone. Molecules that can enter two or more organelles with high selectivity are all but unknown. In this work, we report a naphthalimide-based fluorescent probe, NIM-7, that allows lipid droplets and lysosomes to be labelled simultaneously and with high specificity. These subcellular entities can then be visualized readily through yellow and red fluorescence, using different excitation and detection channels. NIM-7 allows 3D imaging and quantitative visualizing of lipid droplets and lysosomes. It is also able to track simultaneously the movement of lipid droplets and lysosomes in real-time. We also report here that NIM-7 can be used to image both different cell lines and zebrafish embryos.

Graphical abstract: Simultaneous dual-colour tracking lipid droplets and lysosomes dynamics using a fluorescent probe

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Publication details

The article was received on 08 Oct 2018, accepted on 21 Dec 2018 and first published on 27 Dec 2018


Article type: Edge Article
DOI: 10.1039/C8SC04462G
Citation: Chem. Sci., 2019,10, 2342-2348
  • Open access: Creative Commons BY-NC license
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    Simultaneous dual-colour tracking lipid droplets and lysosomes dynamics using a fluorescent probe

    X. Zheng, W. Zhu, F. Ni, H. Ai, S. Gong, X. Zhou, Jonathan L. Sessler and C. Yang, Chem. Sci., 2019, 10, 2342
    DOI: 10.1039/C8SC04462G

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