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A novel strategy for facile serum exosome isolation based on specific interactions between phospholipid bilayers and TiO2

Abstract

Exosomes are cell-derived, phospholipid bilayer-enclosed vesicles that play important roles in intercellular interactions and regulate many biological processes. Accumulating evidence suggests serum exosomes are potential biomarkers for the early diagnosis of cancer. To aid the downstream molecular analyses of tumour-secreted exosomes, purified exosomes are highly desirable. However, current techniques for exosomes isolation are time-consuming and highly instrument-dependent, with limited specificity and recovery. Thus, rapid and efficient methods are strongly needed for both basic research and clinical applications. Here, we present a novel stragety for facile exosome isolation from human serum by taking advantage of the specific interaction of TiO2 with phosphate groups on the lipid bilayer of exosomes. Due to its simplicity and highly affinitive binding, model exosomes can be reversibly isolated with a high recovery (93.4%). Downstream characterization and proteome profiling reveals that high-quality exosomes can be obtained from human serum by this TiO2-based isolation method in 5 min, which is a fraction of the time required for the commonly used ultracentrifugation method. We identified 59 significantly up-regulated proteins by comparing the serum exosomes of pancreatic cancer patients and healthy donors. In addition to the 30 proteins that were reported to be closely related with pancreatic cancer, we found an additional 29 proteins that were had not previously been shown to be related with pancreatic cancer, indicating the potential of this novel method as a powerful tool for exosome isolation for health monitoring and disease diagnosis.

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Publication details

The article was received on 20 Sep 2018, accepted on 30 Nov 2018 and first published on 03 Dec 2018


Article type: Edge Article
DOI: 10.1039/C8SC04197K
Citation: Chem. Sci., 2019, Accepted Manuscript
  • Open access: Creative Commons BY-NC license
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    A novel strategy for facile serum exosome isolation based on specific interactions between phospholipid bilayers and TiO2

    F. gao, F. Jiao, C. Xia, Y. Zhao, W. Ying, Y. Xie, X. Guan, M. Tao, Y. Zhang, W. Qin and X. Qian, Chem. Sci., 2019, Accepted Manuscript , DOI: 10.1039/C8SC04197K

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