Issue 72, 2019, Issue in Progress

A colorimetric nanoprobe based on enzyme-immobilized silver nanoparticles for the efficient detection of cholesterol

Abstract

A large number of cardiovascular diseases have recently become of serious concern throughout the world. Herein, we developed a colorimetric probe based on functionalized silver nanoparticles (AgNPs) for the efficient sensing of cholesterol, an important cardiovascular risk marker. A simple sodium borohydride reduction method was employed to synthesize the AgNPs. The cholesterol oxidase (ChOx)-immobilized AgNPs interact with free cholesterol to produce H2O2 in proportion to the concentration of cholesterol, resulting in decreased AgNP absorbance (turn-off) at 400 nm due to electron transfer between the AgNPs and H2O2. The response of the sensor can also be observed visually. The absorption intensity of the AgNPs is recovered (turn-on) upon the addition of sodium dodecyl sulfate due to the inhibition of ChOx. This on–off mechanism was effectively applied to detect cholesterol within the concentration range 10–250 nM with a low detection limit of approximately 0.014 nM. Moreover, the selectivity of the sensor toward cholesterol was analyzed in the presence of a range of interfering organic substances such as glucose, urea, and sucrose. Finally, the potential of the proposed sensor was evaluated using real samples.

Graphical abstract: A colorimetric nanoprobe based on enzyme-immobilized silver nanoparticles for the efficient detection of cholesterol

Article information

Article type
Paper
Submitted
12 Oct 2019
Accepted
05 Dec 2019
First published
18 Dec 2019
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2019,9, 42085-42095

A colorimetric nanoprobe based on enzyme-immobilized silver nanoparticles for the efficient detection of cholesterol

L. Dewangan, J. Korram, I. Karbhal, R. Nagwanshi, V. K. Jena and M. L. Satnami, RSC Adv., 2019, 9, 42085 DOI: 10.1039/C9RA08328F

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