Samuel Sutiono, Katharina Satzinger, André Pick, Jörg Carsten and Volker Sieber
RSC Adv., 2019,9, 29743-29746
DOI:
10.1039/C9RA06251C,
Paper
Pyruvate decarboxylase (PDC) is a key enzyme for the production of ethanol at high temperatures and for cell-free butanol synthesis. Thermostable, organic solvent stable PDC was evolved from bacterial PDCs. The new variant shows >1500-fold-improved half-life at 75 °C and >5000-fold-increased half-life in the presence of 9 vol% butanol at 50 °C.