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Issue 33, 2019
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Enhanced inertial focusing of microparticles and cells by integrating trapezoidal microchambers in spiral microfluidic channels

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Abstract

In this work, manipulating width and equilibrium position of fluorescent microparticles in spiral microchannel fractionation devices by embedding microchambers along the last turn of a spiral is reported. Microchambers with different shapes and sizes were tested at Reynolds numbers between 15.7 and 156.6 (100–1000 μL min−1) to observe focusing of 2, 5 and 10 μm fluorescent microparticles. This paper also discusses the fabrication process of the microfluidic chips with femtosecond laser ablation on glass wafers, as well as a particle imaging velocimetry (μPIV) study of microparticle trajectories inside a microchamber. It could be demonstrated with an improved final design with inclined microchamber side walls, that the 2 μm particle equilibrium position is shifted towards the inner wall by ∼27 μm and the focusing line's width is reduced by ∼18 μm. Finally, Saccharomyces cerevisiae yeast cells were tested in the final chip and a cell focusing efficiency of 99.1% is achieved.

Graphical abstract: Enhanced inertial focusing of microparticles and cells by integrating trapezoidal microchambers in spiral microfluidic channels

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Publication details

The article was received on 13 May 2019, accepted on 12 Jun 2019 and first published on 18 Jun 2019


Article type: Paper
DOI: 10.1039/C9RA03587G
RSC Adv., 2019,9, 19197-19204
  • Open access: Creative Commons BY-NC license
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    Enhanced inertial focusing of microparticles and cells by integrating trapezoidal microchambers in spiral microfluidic channels

    A. Al-Halhouli, A. Albagdady, W. Al-Faqheri, J. Kottmeier, S. Meinen, L. J. Frey, R. Krull and A. Dietzel, RSC Adv., 2019, 9, 19197
    DOI: 10.1039/C9RA03587G

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