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Issue 38, 2019
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Rapid generation of chemical combinations on a magnetic digital microfluidic array

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Abstract

Combinatorial screening is frequently used to identify chemicals with synergistic effects by measuring the response of biological entities exposed to various chemical-dose combinations. Conventional microwell-based combinatorial screening is resource-demanding, and the closed microfluidics-based screening requires sophisticated fluidic control systems. In this work, we present a novel combinatorial screening platform based on the surface energy trap (SET)-assisted magnetic digital microfluidics. This platform, known as FlipDrop, rapidly generates chemical combinations by coupling two droplet arrays with orthogonal chemical concentration gradients with a simple flip. We have illustrated the working principle of FlipDrop by generating combinations of quantum dots. We have also successfully demonstrated the screening of quantum dot fluorescence resonance energy transfer (QD-FRET) on the FlipDrop platform by measuring the FRET response. This report demonstrates that FlipDrop is capable of rapidly generating chemical combinations with unprecedented ease for combinatorial screening.

Graphical abstract: Rapid generation of chemical combinations on a magnetic digital microfluidic array

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Supplementary files

Article information


Submitted
08 May 2019
Accepted
08 Jul 2019
First published
12 Jul 2019

This article is Open Access

RSC Adv., 2019,9, 21741-21747
Article type
Paper

Rapid generation of chemical combinations on a magnetic digital microfluidic array

Y. Zhang and T. Wang, RSC Adv., 2019, 9, 21741
DOI: 10.1039/C9RA03469B

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

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    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
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    [Original citation] - Published by The Royal Society of Chemistry.

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