Preparation and antimicrobial activity of thyme essential oil microcapsules prepared with gum arabic

Abstract

Thyme essential oils (TEO) exhibit antimicrobial activities against a wide range of pathogenic microorganisms. Microcapsulation technology can be used to improve the stability, water solubility and antibacterial performance of TEO. In this paper, TEO was selected as the core material, and β-cyclodextrin (β-CD) was the wall material for microcapsulation; gum arabic (GA) was used as an emulsifier to prepare microcapsules by coprecipitation. The effects of gum arabic on the encapsulation rate, particle size and release rate of microcapsules were investigated. The optimal condition was found to be TEO : GA by 1 : 3 (w/w) ratio. In this condition, the embedding rate, release rate, and average size of the microcapsules were 87.61%, 53.00%, and 8.20 μm, respectively. Scanning electron microscopy (SEM) revealed that, under the action of gum arabic, the surface of microcapsules was more complete, and the size apparently decreased. Fourier-transform infrared spectroscopy (FTIR) indicated that there was no significant chemical interaction between gum arabic and β-CD. Gum arabic acted only as an emulsifier and remained in the mixed solution. For microcapsules with gum arabic as an emulsifier, the cumulative release rate of essential oils were slower at the initial time compared to microcapsules without added gum arabic. Antimicrobial activity assay exhibited TEO, which showed an inhibitory effect against Botryodiplodia theobromae Pat., and the inhibitory effect was especially strong against Colletotrichum gloeosporioides Penz. Finally, the obtained microcapsules showed the same antibacterial effect.