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Issue 39, 2019
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Processing milk causes the formation of protein oxidation products which impair spatial learning and memory in rats

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Abstract

This study explored the effects of protein oxidation during milk processing on spatial learning and memory in rats. Increasing the heating time, fat content, and inlet air temperature during processing by boiling, microwave heating, spray-drying, or freeze-drying increases milk protein oxidation. Oxidative damage done to milk proteins by microwave heating is greater than that caused by boiling. Dityrosine (DT), as a kind of tyrosine oxidation product, is the most important marker of this process, especially during spray-drying. Rats received diets containing either SWM (spray-dried milk powder diet), FWM (freeze-dried milk powder diet), FWM + LDT (freeze-dried milk powder + low dityrosine diet, DT: 1.4 mg kg−1), or FWM + HDT (freeze-dried milk powder + high dityrosine diet, DT: 2.8 mg kg−1) for 6 weeks. We found that the SWM group, the FWM + LDT group, and the FWM + HDT group appeared to have various degrees of redox state imbalance and oxidative damage in plasma, liver, and brain tissues. Further, hippocampal inflammatory and apoptosis genes were significantly up-regulated in such groups, while learning and memory genes were significantly down-regulated. Eventually, varying degrees of spatial learning and memory impairment were demonstrated in those groups in the Morris water maze. This means that humans should control milk protein oxidation and improve the processing methods applied to food.

Graphical abstract: Processing milk causes the formation of protein oxidation products which impair spatial learning and memory in rats

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Publication details

The article was received on 30 Apr 2019, accepted on 08 Jul 2019 and first published on 17 Jul 2019


Article type: Paper
DOI: 10.1039/C9RA03223A
RSC Adv., 2019,9, 22161-22175
  • Open access: Creative Commons BY-NC license
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    Processing milk causes the formation of protein oxidation products which impair spatial learning and memory in rats

    B. Li, L. Mo, Y. Yang, S. Zhang, J. Xu, Y. Ge, Y. Xu, Y. Shi and G. Le, RSC Adv., 2019, 9, 22161
    DOI: 10.1039/C9RA03223A

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