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Issue 32, 2019, Issue in Progress
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Microfabricated needle for hydrogen peroxide detection

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Abstract

A microfabricated needle-like probe has been designed and applied for hydrogen peroxide (H2O2) sampling and detection using a commercial, single-step fluorescent H2O2 assay. In this work, droplets of the assay reagent are generated and sent to the needle tip using a mineral-oil carrier fluid. At the needle tip, the sample is drawn into the device through 100 μm long hydrophilic capillaries by negative pressure. The sampled fluid is immediately merged with the assay droplet and carried away to mix and react, producing a sequence of droplets representing the H2O2 concentration as a function of time. We have characterized the assay fluorescence for small variations in the sample volume. With the calibration, we can calculate the concentration of H2O2 in the sampled liquid from the size and intensity of each merged droplet. This is a microfluidic data-logger system for on-site continuous sampling, controlled reaction, signal storage and on-line quantitative detection. It is a useful tool for monitoring dynamic chemical reactions in analytical chemistry and biological applications.

Graphical abstract: Microfabricated needle for hydrogen peroxide detection

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Publication details

The article was received on 23 Apr 2019, accepted on 26 May 2019 and first published on 10 Jun 2019


Article type: Paper
DOI: 10.1039/C9RA03028J
RSC Adv., 2019,9, 18176-18181
  • Open access: Creative Commons BY-NC license
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    Microfabricated needle for hydrogen peroxide detection

    S. Feng, S. Clement, Y. Zhu, Ewa M. Goldys and D. W. Inglis, RSC Adv., 2019, 9, 18176
    DOI: 10.1039/C9RA03028J

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