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Issue 14, 2019
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A lysosome-targetable fluorescent probe for the simultaneous sensing of Cys/Hcy and GSH from different emission channels

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Abstract

A lysosome-specific fluorescent probe, Lyso-AC, for biothiols was developed by incorporation of a 4-nitrophenol moiety into a coumarin dye. The Cys/Hcy-triggered substitution-rearrangement cascade, and GSH-induced substitution reaction lead to the corresponding blue emissive amino-coumarin and yellow emissive thiol-coumarin, thereby enabling Cys/Hcy and GSH detection from distinct emissions. Moreover, this probe displayed an excellent lysosome targeting property with a 0.92 Pearson's colocalization coefficient by using Neutral Red as a reference. Significantly, biological experiments indicated Lyso-AC has the potential to monitor lysosome Cys/Hcy and GSH simultaneously in living HeLa cells from distinct emissions.

Graphical abstract: A lysosome-targetable fluorescent probe for the simultaneous sensing of Cys/Hcy and GSH from different emission channels

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Supplementary files

Article information


Submitted
10 Jan 2019
Accepted
25 Feb 2019
First published
12 Mar 2019

This article is Open Access

RSC Adv., 2019,9, 7955-7960
Article type
Paper

A lysosome-targetable fluorescent probe for the simultaneous sensing of Cys/Hcy and GSH from different emission channels

H. Zhang, L. Xu, W. Li, W. Chen, Q. Xiao, J. Huang, C. Huang, J. Sheng and X. Song, RSC Adv., 2019, 9, 7955
DOI: 10.1039/C9RA00210C

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