Jump to main content
Jump to site search

Issue 13, 2019, Issue in Progress
Previous Article Next Article

Migration of mesenchymal stem cells tethered with carbon nanotubes under a chemotactic gradient

Author affiliations

Abstract

Carbon nanotubes (CNTs) have been extensively studied for photothermal ablation of malignant cells due to their ability to absorb near-infrared (NIR) laser light and convert it to thermal energy for the lysis of tumor cells. Functionalizing CNTs with tumor-targeting moieties can facilitate the delivery to tumor sites. Instead of using targeting moieties, mesenchymal stem cells (MSCs) have been considered as vehicles to deliver therapeutic agents to cancer cells. In this study, the effects of attaching CNTs to MSCs on cell migration in response to a chemotactic gradient were investigated. Multiwalled carbon nanotubes (MWCNTs) were functionalized with streptavidin–fluorescein isothiocyanate (SA–FITC). The surface of human MSCs was biotinylated by culturing MSCs with biotin–lipid containing medium. CNTs were then attached on the outer cell membrane of biotinylated MSCs through SA–biotin binding. Fluorescence microscopy confirmed CNTs were located on the surface of MSCs. Various amounts of CNTs anchored on the membrane of MSCs were used to examine the effects of CNTs on MSC proliferation and migration. Our transwell migration assay showed that 4.26 ng CNT per cell is the threshold value that would not affect the migration speed of CNT-tagged MSCs toward the established gradient of chemoattractant SDF-1α.

Graphical abstract: Migration of mesenchymal stem cells tethered with carbon nanotubes under a chemotactic gradient

Back to tab navigation

Article information


Submitted
27 Nov 2018
Accepted
24 Feb 2019
First published
01 Mar 2019

This article is Open Access

RSC Adv., 2019,9, 7156-7164
Article type
Paper

Migration of mesenchymal stem cells tethered with carbon nanotubes under a chemotactic gradient

J. Zhang and C. Peng, RSC Adv., 2019, 9, 7156
DOI: 10.1039/C8RA09768B

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

Reproduced material should be attributed as follows:

  • For reproduction of material from NJC:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
  • For reproduction of material from PCCP:
    [Original citation] - Published by the PCCP Owner Societies.
  • For reproduction of material from PPS:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
  • For reproduction of material from all other RSC journals:
    [Original citation] - Published by The Royal Society of Chemistry.

Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.


Social activity

Search articles by author

Spotlight

Advertisements