Jump to main content
Jump to site search

Issue 1, 2019
Previous Article Next Article

Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells

Author affiliations

Abstract

We developed a fast strain-promoted azide–alkyne cycloaddition reaction (SPAAC) by tetra-fluorinated aromatic azide with a kinetic constant of 3.60 M−1 s−1, which is among the fastest SPAAC ligations reported so far. We successfully employed the reaction for covalent labelling of proteins with high efficiency and for bioimaging of mitochondria in living cells. The reaction could be a generally useful toolbox for chemical biology and biomaterials.

Graphical abstract: Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells

Back to tab navigation

Supplementary files

Article information


Submitted
11 Nov 2018
Accepted
10 Dec 2018
First published
19 Dec 2018

This article is Open Access

RSC Adv., 2019,9, 23-26
Article type
Paper

Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells

X. Cai, D. Wang, Y. Gao, L. Yi, X. Yang and Z. Xi, RSC Adv., 2019, 9, 23
DOI: 10.1039/C8RA09303B

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

Reproduced material should be attributed as follows:

  • For reproduction of material from NJC:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
  • For reproduction of material from PCCP:
    [Original citation] - Published by the PCCP Owner Societies.
  • For reproduction of material from PPS:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
  • For reproduction of material from all other RSC journals:
    [Original citation] - Published by The Royal Society of Chemistry.

Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.


Social activity

Search articles by author

Spotlight

Advertisements