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Issue 7, 2019
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Insight into the effect of quinic acid on biofilm formed by Staphylococcus aureus

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Abstract

The biofilm formation of Staphylococcus aureus on food contact surfaces is the main risk of food contamination. In the present study, we firstly investigated the inhibitory effect of quinic acid (QA) on biofilm formed by S. aureus. Crystal violet staining assay and microscopy analysis clearly showed that QA at sub-MIC concentrations was able to significantly reduce the biofilm biomass and cause a collapse on biofilm architecture. Meanwhile, fibrinogen binding assay showed that QA had obviously effect on the S. aureus bacteria adhesion. XTT reduction assay and confocal laser scanning microscopic images revealed that QA significantly decreased metabolic activity and viability of biofilm cells. In addition, qRT-PCR analysis explored the potential inhibitory mechanism of QA against biofilm formation, which indicated that QA significantly repressed the gene sarA and activated the gene agrA. Moreover, QA exhibited a highly ability to reduce the number of sessile S. aureus cells adhered on the stainless steel. So, it was suggested that QA could be used as a promising antibiofilm agent to control biofilm formation of S. aureus.

Graphical abstract: Insight into the effect of quinic acid on biofilm formed by Staphylococcus aureus

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Article information


Submitted
04 Nov 2018
Accepted
22 Jan 2019
First published
29 Jan 2019

This article is Open Access

RSC Adv., 2019,9, 3938-3945
Article type
Paper

Insight into the effect of quinic acid on biofilm formed by Staphylococcus aureus

J. Bai, Y. Wu, G. Elena, K. Zhong and H. Gao, RSC Adv., 2019, 9, 3938
DOI: 10.1039/C8RA09136F

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    [Original citation] - Published by The Royal Society of Chemistry.

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