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Issue 20, 2019
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Culturing and patch clamping of Jurkat T cells and neurons on Al2O3 coated nanowire arrays of altered morphology

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Abstract

Nanowire substrates play an increasingly important role for cell cultures as an approach for hybrid bio-semiconductor junctions. We investigate Jurkat T cells and neurons from mice cultured on Al2O3 coated ordered and randomly distributed nanowires. Cell viability was examined by life/membrane staining reporting comparable viability on planar and nanowire substrates. Imaging the hybrid interface reveals a wrapping of the cell membrane around the very nanowire tip. Patch clamp recordings show similar electrophysiological responses on each type of nanowires compared to planar control substrates. We demonstrate that the morphological characteristic of the nanowire substrate plays a subordinate role which opens up the arena for a large range of nanowire substrates in a functionalized application such as stimulation or sensing.

Graphical abstract: Culturing and patch clamping of Jurkat T cells and neurons on Al2O3 coated nanowire arrays of altered morphology

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Publication details

The article was received on 21 Jun 2018, accepted on 30 Mar 2019 and first published on 09 Apr 2019


Article type: Paper
DOI: 10.1039/C8RA05320K
RSC Adv., 2019,9, 11194-11201
  • Open access: Creative Commons BY-NC license
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    Culturing and patch clamping of Jurkat T cells and neurons on Al2O3 coated nanowire arrays of altered morphology

    J. Harberts, R. Zierold, C. Fendler, A. Koitmäe, P. Bayat, I. Fernandez-Cuesta, G. Loers, B. Diercks, R. Fliegert, A. H. Guse, C. Ronning, G. Otnes, M. Borgström and R. H. Blick, RSC Adv., 2019, 9, 11194
    DOI: 10.1039/C8RA05320K

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