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Preparation of A Novel Sandwich-Type Electrochemical Immunosensor for AFP Detection Based on ATRP and Click Chemistry Technique

Abstract

It is extremely important to explore the synthesis methodology and application scope of functional polymer brushes-based nanocomposites. In this work, an ultrasensitive sandwich-type electrochemical immunosensor based on polymer brush/graphene oxide (GO) nanoprobe was designed for quantitative detection of alpha fetoprotein (AFP). As the backbone of the polymer brush, polyacrylonitrile-g-GO (PAN-g-GO) was efficiently prepared via surface-initiated atom transfer radical polymerization (SI-ATRP). Benefit from the large number of nitrile groups in PAN, the poly (vinyltetrazole-co-hydroxyethyl methacrylate)-g-GO (P(VT-co-HEMA)-g-GO) was synthesized herein through nitrile click chemistry. Furthermore, the immunosensor reached the design requirements of high sensitivity through the P(VT-co-HEMA)-g-GO connected to plenty of signal labels. Importantly, the designed immunosensor showed a wide range (2.5×101-5×104 pg/mL) (R2 = 0.993) and a low detection limit (LOD, ~ 0.183 pg/mL) with high selectivity, good reproducibility and excellent stability. Therefore, these polymer brushes-based nanocomposites can provide potential applications for determining tumor markers (TMs) with robust signal amplification’s capability.

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Publication details

The article was received on 26 Aug 2019, accepted on 27 Nov 2019 and first published on 28 Nov 2019


Article type: Paper
DOI: 10.1039/C9PY01279F
Polym. Chem., 2019, Accepted Manuscript

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    Preparation of A Novel Sandwich-Type Electrochemical Immunosensor for AFP Detection Based on ATRP and Click Chemistry Technique

    X. Zhao, N. Wang, H. Chen, L. Bai, H. Xu, W. Wang, H. Yang, D. Wei, L. Yang and Z. Cheng, Polym. Chem., 2019, Accepted Manuscript , DOI: 10.1039/C9PY01279F

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