Jump to main content
Jump to site search


Synthesis of an ortho-phthalaldehyde-functionalized copolymer for rapid, chemoselective and efficient conjugation with native proteins under physiological conditions

Author affiliations

Abstract

We report the synthesis of an ortho-phthalaldehyde (OPA) functionalized and water-soluble copolymer by reversible addition-fragmentation chain transfer (RAFT) copolymerization of a novel OPA derivative vinyl monomer with N,N-dimethylacrylamide, followed by deprotection in trifluoroacetic acid/dichloromethane solution. Our results demonstrate that the OPA-containing copolymer could conjugate rapidly, chemoselectively and efficiently with native proteins via the OPA-primary amine reaction under physiological conditions without significant changes in the protein activity. This work provides a promising method for efficient conjugation of polymers with native proteins under physiological conditions.

Graphical abstract: Synthesis of an ortho-phthalaldehyde-functionalized copolymer for rapid, chemoselective and efficient conjugation with native proteins under physiological conditions

Back to tab navigation

Publication details

The article was received on 06 Mar 2019, accepted on 12 Apr 2019 and first published on 12 Apr 2019


Article type: Paper
DOI: 10.1039/C9PY00365G
Citation: Polym. Chem., 2019, Advance Article

  •   Request permissions

    Synthesis of an ortho-phthalaldehyde-functionalized copolymer for rapid, chemoselective and efficient conjugation with native proteins under physiological conditions

    J. Huang, H. Qin, X. Chen, B. Wang, H. Liang and J. Lu, Polym. Chem., 2019, Advance Article , DOI: 10.1039/C9PY00365G

Search articles by author

Spotlight

Advertisements