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Synthesis of ortho-phthalaldehyde-functionalized copolymer for rapid, chemoselective and efficient conjugation with native proteins under physiological condition

Abstract

We report the synthesis of Ortho-phthalaldehyde (OPA) functionalized and water-soluble copolymer by reversible addition-fragmentation chain transfer (RAFT) copolymerization of a novel OPA derivative vinyl monomer with N,N-dimethylacrylamide, followed by deprotection in trifluoroacetic acid/ dichloromethane solution. Our results demonstrate that the OPA-containing copolymer could conjugate rapidly, chemoselectively and efficiently with native proteins via OPA-primary amine reaction under physiological condition without significant change of the protein activity. This work provides a promising method for efficient conjugation of polymers with native proteins under physiological condition.

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Publication details

The article was received on 06 Mar 2019, accepted on 12 Apr 2019 and first published on 12 Apr 2019


Article type: Paper
DOI: 10.1039/C9PY00365G
Citation: Polym. Chem., 2019, Accepted Manuscript

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    Synthesis of ortho-phthalaldehyde-functionalized copolymer for rapid, chemoselective and efficient conjugation with native proteins under physiological condition

    J. Huang, H. Qin, X. Chen, B. Wang, H. Liang and J. Lu, Polym. Chem., 2019, Accepted Manuscript , DOI: 10.1039/C9PY00365G

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