Issue 23, 2019

Site-specific conjugation of antifreeze proteins onto polymer-stabilized nanoparticles

Abstract

Antifreeze proteins (AFPs) have many potential applications, ranging from cryobiology to aerospace, if they can be incorporated into materials. Here, a range of engineered AFP mutants were prepared and site-specifically conjugated onto RAFT polymer-stabilized gold nanoparticles to generate new hybrid multivalent ice growth inhibitors. Only the SNAP-tagged AFPs lead to potent ‘antifreeze’ active nanomaterials with His-Tag capture resulting in no activity, showing the mode of conjugation is essential. This versatile strategy will enable the development of multivalent AFPs for translational and fundamental studies.

Graphical abstract: Site-specific conjugation of antifreeze proteins onto polymer-stabilized nanoparticles

Supplementary files

Article information

Article type
Communication
Submitted
03 Dec 2018
Accepted
21 Jan 2019
First published
31 Jan 2019
This article is Open Access
Creative Commons BY license

Polym. Chem., 2019,10, 2986-2990

Site-specific conjugation of antifreeze proteins onto polymer-stabilized nanoparticles

L. E. Wilkins, M. Hasan, A. E. R. Fayter, C. Biggs, M. Walker and M. I. Gibson, Polym. Chem., 2019, 10, 2986 DOI: 10.1039/C8PY01719K

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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