Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 10, 2019
Previous Article Next Article

Correlation between polymer architecture and polyion complex micelle stability with proteins in spheroid cancer models as seen by light-sheet microscopy

Author affiliations

Abstract

Polyion complex (PIC) micelles are frequently used as a means to deliver biologics such as proteins. While it is known that the polymer structure can affect the stability of these micelles, few studies have investigated their stability in biological settings. In this paper, we have prepared a library of poly[poly(ethylene glycol) methyl ether acrylate]-block-poly(2-carboxyethyl acrylate)s (PPEGMEA-b-PCEA) and poly[poly(ethylene glycol) methyl ether acrylate]-block-poly(acrylic acid)s (PPEGMEA-b-PAA), each with varying chain length of the charged polymer block. In addition terpolymers were prepared that included hydrophobic n-butyl acrylate (BA) units randomly incorporated along the charged block and as triblock terpolymers (PPEGMEA-b-(PCEA-co-PBA), PPEGMEA-b-PCEA-b-PBA or PPEGMEA-b-PBA-b-PCEA). These polymers were condensed with lysozyme to generate PIC micelles of various compositions. While stable in the intracellular space, the diblock PIC micelles readily disassembled inside the cells of spheroid cancer models as evidenced by light-sheet fluorescence microscopy (LSFM) and Förster resonance energy transfer (FRET) studies. Terpolymer PIC micelles were more resistant to disassembly, but only when the hydrophobic BA groups were randomly distributed within the pCEA blocks and not when incorporated as a separate block. These results show that simple PIC micelles will rapidly deliver biological cargo after cell internalization and their stability can be tuned by the introduction of hydrophobic units within the charged block.

Graphical abstract: Correlation between polymer architecture and polyion complex micelle stability with proteins in spheroid cancer models as seen by light-sheet microscopy

Back to tab navigation

Supplementary files

Article information


Submitted
04 Nov 2018
Accepted
17 Jan 2019
First published
24 Jan 2019

Polym. Chem., 2019,10, 1221-1230
Article type
Paper

Correlation between polymer architecture and polyion complex micelle stability with proteins in spheroid cancer models as seen by light-sheet microscopy

F. Chen, R. Raveendran, C. Cao, R. Chapman and M. H. Stenzel, Polym. Chem., 2019, 10, 1221
DOI: 10.1039/C8PY01565A

Social activity

Search articles by author

Spotlight

Advertisements