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Excited State Proton Transfer Based Fluorescent Molecular Probes and their Application in Studying Lipid Bilayer Membranes

Abstract

Molecules with ionizable protons, with different proton transfer efficiencies in the excited and ground states, show excited state prototropism (ESPT). In suitable proton donating/accepting environments, ESPT of a molecule can result in the observation of emissions from different prototropic species, each characterized by different emission spectra and different emission lifetimes. In condensed media, the immediate environment around the ESPT molecule can significantly influence the emission spectral parameters of different prototropic species. This forms the basis of ESPT based fluorescence sensing. The concept of ESPT has been widely used in probing dynamical and structural information of micro-heterogeneous media like micelles, polymers, lipid bilayer membranes, etc. ESPT molecules like naphthol and intra-molecular ESPT (ESIPT) molecules like hydroxyflavones etc., are said to be good multi-state fluorescent molecular probes if (i) the partitioning of these molecules to a micro-heterogeneous organized medium is more efficient, and (ii) the molecules possess distinct excitation and emission wavelengths corresponding to its different prototropic forms. The fluorescence of different prototropic forms shows sensitive response towards the change in the local environment around the micro-heterogeneous organized medium concerning the physical properties, local structure, and dynamics. This review mainly comprises the work carried out on ESPT fluorescence molecular probing of biomimetic liposomes/lipid bilayer membranes from 1990 onwards.

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Publication details

The article was received on 12 Jul 2019, accepted on 30 Oct 2019 and first published on 07 Nov 2019


Article type: Perspective
DOI: 10.1039/C9PP00294D
Photochem. Photobiol. Sci., 2019, Accepted Manuscript

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    Excited State Proton Transfer Based Fluorescent Molecular Probes and their Application in Studying Lipid Bilayer Membranes

    A. K. Mishra and M. MOHAPATRA, Photochem. Photobiol. Sci., 2019, Accepted Manuscript , DOI: 10.1039/C9PP00294D

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