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Issue 5, 2019
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The dephosphorylated S8A and S18A mutants of (oat) phytochrome A comprise its two species, phyA′ and phyA′′, suggesting that autophosphorylation at these sites is not involved in the phyA differentiation

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Abstract

Phytochrome A (phyA) is represented in plants by two species, phyA′ and phyA′′, with different properties and modes of action (Sineshchekov, Funct. Plant Biol., 2019, 46, 118–135). They differ by the modification of a serine(s) residue at the N-terminus, possibly, by phosphorylation. To verify if these serines could be the Ser8 and Ser18 (in Avena sativa phyA, AsphyA), whose autophosphorylation modulates AsphyA stability and sensitivity as shown with the use of the serine-to-alanine substitution AsphyA mutants (S8A, S18A and S8/18A) (Han et al., Plant Cell Physiol., 2010, 51, 596–609), we have undertaken low-temperature (85 K) fluorescence investigations of phyA in these transgenic lines. The content and proportion of phyA′ and phyA′′ were essentially the same in wild-type AsphyA and its mutants, and in endogenous Arabidopsis phyA. All the lines revealed a higher phyA′/phyA′′ proportion upon longer germination-inducing preillumination (3 h vs. 15 min white light) supporting our earlier finding that the phyA differentiation into the subpools is light-regulated. These observations and our earlier data imply that this process involves N-terminal serine(s) different from the autophosphorylated Ser8 and Ser18 (in AsphyA) narrowing down the area of further search for the exact site(s) of the phyA modification.

Graphical abstract: The dephosphorylated S8A and S18A mutants of (oat) phytochrome A comprise its two species, phyA′ and phyA′′, suggesting that autophosphorylation at these sites is not involved in the phyA differentiation

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Publication details

The article was received on 15 Dec 2018, accepted on 28 Feb 2019 and first published on 28 Feb 2019


Article type: Paper
DOI: 10.1039/C8PP00574E
Photochem. Photobiol. Sci., 2019,18, 1242-1248

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    The dephosphorylated S8A and S18A mutants of (oat) phytochrome A comprise its two species, phyA′ and phyA′′, suggesting that autophosphorylation at these sites is not involved in the phyA differentiation

    V. Sineshchekov, L. Koppel and J.-I. Kim, Photochem. Photobiol. Sci., 2019, 18, 1242
    DOI: 10.1039/C8PP00574E

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