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A trimethyllysine-containing trityl tag for solubilizing hydrophobic peptides

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Abstract

Hydrophobic membrane peptides/proteins having low water solubility are often difficult to prepare. To overcome this issue, temporal introduction of solubilizing tags has been demonstrated to be beneficial. Following our recent work on the solubilization of a difficult target by using a hydrophilic oligo-Lys tag bearing a trityl linker (Trt-K method), this paper describes a comparative study of the solubilizing abilities of several peptidic trityl tags containing Lys, Arg, Glu, Asn, Nε-tri-Me-Lys or Cys-sulfonate using two hydrophobic model peptides. Among the tags evaluated, that containing Nε-tri-Me-Lys exhibits superior solubilizing ability.

Graphical abstract: A trimethyllysine-containing trityl tag for solubilizing hydrophobic peptides

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Publication details

The article was received on 17 Oct 2019, accepted on 20 Nov 2019 and first published on 21 Nov 2019


Article type: Paper
DOI: 10.1039/C9OB02253H
Org. Biomol. Chem., 2019, Advance Article

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    A trimethyllysine-containing trityl tag for solubilizing hydrophobic peptides

    S. Masuda, S. Tsuda and T. Yoshiya, Org. Biomol. Chem., 2019, Advance Article , DOI: 10.1039/C9OB02253H

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