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Total chemical synthesis of murine ISG15 and an activity-based probe with physiological binding properties

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Abstract

The linear synthesis of the N-terminal domain of mISG15 has been developed which enables the synthesis of full-length mISG15 and the activity-based probe Rho-mISG15-PA via native chemical ligation. Pilot experiments showed that the synthetic proteins were properly folded and recognized by endogenous enzymes. Our synthesis strategy allows the generation of other mISG15-based probes and reagents that can accelerate the research in this field.

Graphical abstract: Total chemical synthesis of murine ISG15 and an activity-based probe with physiological binding properties

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Publication details

The article was received on 01 Oct 2019, accepted on 30 Oct 2019 and first published on 31 Oct 2019


Article type: Communication
DOI: 10.1039/C9OB02127B
Org. Biomol. Chem., 2019, Advance Article
  • Open access: Creative Commons BY-NC license
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    Total chemical synthesis of murine ISG15 and an activity-based probe with physiological binding properties

    B. Xin, G. Jin, D. J. Fernandez, K. Knobeloch, P. P. Geurink and H. Ovaa, Org. Biomol. Chem., 2019, Advance Article , DOI: 10.1039/C9OB02127B

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