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Issue 44, 2019
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A ratiometric fluorescent probe for simultaneous detection of Cys/Hcy and GSH

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Abstract

GSH, Cys and Hcy are the main intracellular thiols to play crucial roles in human pathologies. It is a great challenge to differentiate these three biothiols using single molecular fluorescent probes due to their close similarities in chemical structure and reactivity. In this work, based on the fluorescence resonance energy transfer (FRET) mechanism, a fluorescent probe CPR was constructed to simultaneously distinguish GSH and Cys/Hcy by means of ratiometric fluorescence changes: from red (584 nm) to green (542 nm) for GSH and from red (584 nm) to blue (472 nm) for Cys/Hcy. This probe showed high sensitivity and selectivity with low limits of detection (LOD = 12 nm, 13 nm and 30 nm for Cys, Hcy and GSH, respectively) and was capable of imaging GSH and Cys/Hcy in cells and zebrafish in a ratiometric manner with low toxicity.

Graphical abstract: A ratiometric fluorescent probe for simultaneous detection of Cys/Hcy and GSH

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Publication details

The article was received on 07 Sep 2019, accepted on 22 Oct 2019 and first published on 30 Oct 2019


Article type: Paper
DOI: 10.1039/C9OB01960J
Org. Biomol. Chem., 2019,17, 9631-9635

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    A ratiometric fluorescent probe for simultaneous detection of Cys/Hcy and GSH

    H. Zhang, B. Wang, Y. Ye, W. Chen and X. Song, Org. Biomol. Chem., 2019, 17, 9631
    DOI: 10.1039/C9OB01960J

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