Jump to main content
Jump to site search

Issue 24, 2019

Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases

Author affiliations

Abstract

Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprecedented reversible activity of bacterial β1,4-galactosyltransferases to catalyse the transgalactosylation from lactose to N-acetylglucosamine to form N-acetyllactosamine in the presence of UDP. The process is demonstrated by the preparative scale synthesis of pNP-β-LacNAc from lactose using β1,4-galactosyltransferase NmLgtB-B as the only biocatalyst.

Graphical abstract: Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases

Supplementary files

Article information


Submitted
10 May 2019
Accepted
29 May 2019
First published
30 May 2019

Org. Biomol. Chem., 2019,17, 5920-5924
Article type
Communication

Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases

K. Huang, F. Parmeggiani, H. Ledru, K. Hollingsworth, J. Mas Pons, A. Marchesi, P. Both, A. P. Mattey, E. Pallister, G. S. Bulmer, J. M. van Munster, W. B. Turnbull, M. C. Galan and S. L. Flitsch, Org. Biomol. Chem., 2019, 17, 5920 DOI: 10.1039/C9OB01089K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements