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Issue 24, 2019
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Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases

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Abstract

Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprecedented reversible activity of bacterial β1,4-galactosyltransferases to catalyse the transgalactosylation from lactose to N-acetylglucosamine to form N-acetyllactosamine in the presence of UDP. The process is demonstrated by the preparative scale synthesis of pNP-β-LacNAc from lactose using β1,4-galactosyltransferase NmLgtB-B as the only biocatalyst.

Graphical abstract: Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases

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Supplementary files

Article information


Submitted
10 May 2019
Accepted
29 May 2019
First published
30 May 2019

Org. Biomol. Chem., 2019,17, 5920-5924
Article type
Communication

Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases

K. Huang, F. Parmeggiani, H. Ledru, K. Hollingsworth, J. Mas Pons, A. Marchesi, P. Both, A. P. Mattey, E. Pallister, G. S. Bulmer, J. M. van Munster, W. B. Turnbull, M. C. Galan and S. L. Flitsch, Org. Biomol. Chem., 2019, 17, 5920
DOI: 10.1039/C9OB01089K

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