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Issue 25, 2019
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Rational design of a function-based selection method for genetically encoding acylated lysine derivatives

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Abstract

A foundation of genetic code expansion is the evolution of orthogonal aminoacyl-tRNA synthetase to recognize a non-canonical amino acid, which typically involves read-through of amber stop codon in the genes used in selection. The process is time-consuming, labor-intensive, and susceptible to certain bias. As a complementation, herein, we rationally designed a function-based method to directed evolution of aminoacyl-tRNA synthetase for acylated lysine derivatives.

Graphical abstract: Rational design of a function-based selection method for genetically encoding acylated lysine derivatives

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Publication details

The article was received on 30 Apr 2019, accepted on 04 Jun 2019 and first published on 05 Jun 2019


Article type: Communication
DOI: 10.1039/C9OB00992B
Org. Biomol. Chem., 2019,17, 6127-6130

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    Rational design of a function-based selection method for genetically encoding acylated lysine derivatives

    H. Miao, C. Yu, A. Yao and W. Xuan, Org. Biomol. Chem., 2019, 17, 6127
    DOI: 10.1039/C9OB00992B

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