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Arginine mimetic appended peptide-based probes for fluorescence turn-on detection of 14-3-3 proteins

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Abstract

14-3-3 proteins are adaptor elements in intracellular signaling pathways. Recently, this protein family has been identified as a relevant therapeutic target involved in many human diseases. Therefore, identification of 14-3-3 proteins in biological systems is very important. Two cationic peptide-based probes are reported for the fluorescence detection of 14-3-3 proteins at physiological pH. The design of these probes consists of two symmetric peptidic arms equipped with a guanidiniocarbonyl pyrrole moiety (an arginine mimetic aka GCP), and an environment-sensitive amino-naphthalimide fluorophore as a third arm. These peptide sequences also contain lysine and phenylalanine/tryptophan amino acids for additional charge–charge and hydrophobic interactions. Both probes show high affinity and sensitivity for the 14-3-3 family, as well as good selectivity against other relevant biological proteins and ions.

Graphical abstract: Arginine mimetic appended peptide-based probes for fluorescence turn-on detection of 14-3-3 proteins

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Publication details

The article was received on 26 Mar 2019, accepted on 26 Mar 2019 and first published on 02 Apr 2019


Article type: Paper
DOI: 10.1039/C9OB00620F
Citation: Org. Biomol. Chem., 2019, Advance Article

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    Arginine mimetic appended peptide-based probes for fluorescence turn-on detection of 14-3-3 proteins

    D. Maity, A. Gigante, P. A. Sánchez-Murcia, E. Sijbesma, M. Li, D. Bier, S. Mosel, S. Knauer, C. Ottmann and C. Schmuck, Org. Biomol. Chem., 2019, Advance Article , DOI: 10.1039/C9OB00620F

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