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Detection and identification of O-GlcNAc-modified proteins using 6-azido-6-deoxy-N-acetyl-galactosamine

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Abstract

An unnatural monosaccharide with a C6-azide, Ac36AzGalNAc, has been developed as a potent and selective probe for O-GlcNAc-modified proteins. Combined with click chemistry, we demonstrate that Ac36AzGalNAc can robustly label O-GlcNAc glycosylation in a wide range of cell lines. Meanwhile, cell imaging and LC-MS/MS proteomics verify its selective activity on O-GlcNAc. More importantly, the protocol presented here provides a general methodology for tracking, capturing and identifying unnatural monosaccharide modified proteins in cells or cell lysates.

Graphical abstract: Detection and identification of O-GlcNAc-modified proteins using 6-azido-6-deoxy-N-acetyl-galactosamine

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Publication details

The article was received on 02 Mar 2019, accepted on 04 Apr 2019 and first published on 06 Apr 2019


Article type: Paper
DOI: 10.1039/C9OB00516A
Citation: Org. Biomol. Chem., 2019, Advance Article

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    Detection and identification of O-GlcNAc-modified proteins using 6-azido-6-deoxy-N-acetyl-galactosamine

    J. Guo, G. Zhang, J. Ma, C. Zhao, Q. Xue, J. Wang, W. Liu, K. Liu, H. Wang, N. Liu, Q. Song and J. Li, Org. Biomol. Chem., 2019, Advance Article , DOI: 10.1039/C9OB00516A

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